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KMID : 0363020090390010077
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Journal of Korean Academy of Periodontology
2009 Volume.39 No. 1 p.77 ~ p.86
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Effect of rhBMP-2 produced by Escherichia coli expression system on bone formation in rat calvarial defects
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Kwon Suk-Hoon
Jung Ui-Won
Choi Seong-Ho
Lim Hyun-Chang
Kim Chang-Sung
Kim Min-Soo
Yun Jeong-Ho
Choi Kyung-Hee
Cho Kyoo-Sung
Lee Ji-Hyun
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Abstract
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Purpose: Recombinant human bone morphogenetic protein-2(rhBMP-2) has been evaluated as potential candidates for periodontal and bone regenerative therapy. In spite of good prospects in BMP applications, there is economically unavailable for clinical use in dental area. The purpose of this study was to evaluate the osteogenic effect of rhBMP-2 produced by E.coli expression system.
Materials and methods: Eight-mm critical-size calvarial defects were created in 48 male Sprague-Dawley rats. The animals were divided into 6 groups of 8 animals each. Each group received one of the following: Negative control(sham-surgery control), positive control(absorbable collagen sponge(ACS) alone) and experimental(ACS loaded with rhBMP-2). Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals.
Results: The experimental group showed significant defect closure at 2 and 8weeks than the sham surgery and positive control groups. Moreover, the experimental group showed significantly greater new bone and augmented area than the other groups at both 2 and 8weeks.
Conclusion: rhBMP-2 produced by E.coli expression system may be effective for bone regeneration.
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KEYWORD
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Bone morphogenetic protein-2, E.coli expression system, Absorbable collagen sponge, rat calvarial defect
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